rabbit antinos polyclonal ab Search Results


rabbit antinf kb primary antibody  (Santa Cruz Biotechnology)
99
Santa Cruz Biotechnology rabbit antinf kb primary antibody
Rabbit Antinf Kb Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/pm30055890-78-7-11?v=Santa+Cruz+Biotechnology
Average 99 stars, based on 1 article reviews
rabbit antinf kb primary antibody - by Bioz Stars, 2026-08
99/100 stars
  Buy from Supplier
rabbit monoclonal antin-terminal ndrg1 (amino acids 19-48  (WuXi AppTec)
90
WuXi AppTec rabbit monoclonal antin-terminal ndrg1 (amino acids 19-48
Rabbit Monoclonal Antin Terminal Ndrg1 (Amino Acids 19 48, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/pm29679718-66-20-31?v=WuXi+AppTec
Average 90 stars, based on 1 article reviews
rabbit monoclonal antin-terminal ndrg1 (amino acids 19-48 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
rabbit antinf κb p65 antibody  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rabbit antinf κb p65 antibody
Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB <t>p65</t> nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.
Rabbit Antinf κb P65 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/pm20878086-91-6-26?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
rabbit antinf κb p65 antibody - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
rabbit antina k atpase  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology rabbit antina k atpase
Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB <t>p65</t> nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.
Rabbit Antina K Atpase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/pm31064810-56-23-35?v=Santa+Cruz+Biotechnology
Average 95 stars, based on 1 article reviews
rabbit antina k atpase - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
antinf kb  (Servicebio Inc)
86
Servicebio Inc antinf kb
Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB <t>p65</t> nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.
Antinf Kb, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/pm41239505-96-30-33?v=Servicebio+Inc
Average 86 stars, based on 1 article reviews
antinf kb - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
anti-nnos antibody  (Euro Diagnostica)
90
Euro Diagnostica anti-nnos antibody
Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB <t>p65</t> nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.
Anti Nnos Antibody, supplied by Euro Diagnostica, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/pm11375331-91-11-27?v=Euro+Diagnostica
Average 90 stars, based on 1 article reviews
anti-nnos antibody - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
rabbit antin6-methyladenosine (m6a)  (Merck KGaA)
90
Merck KGaA rabbit antin6-methyladenosine (m6a)
Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB <t>p65</t> nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.
Rabbit Antin6 Methyladenosine (M6a), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/10__7554_slash_elife__68312-408-23-26?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
rabbit antin6-methyladenosine (m6a) - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
rabbit antina pab  (GeneTex)
90
GeneTex rabbit antina pab
Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB <t>p65</t> nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.
Rabbit Antina Pab, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/pm35377920-298-117-121?v=GeneTex
Average 90 stars, based on 1 article reviews
rabbit antina pab - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
rabbit anti nuclear factor kappa b  (Proteintech)
97
Proteintech rabbit anti nuclear factor kappa b
Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB <t>p65</t> nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.
Rabbit Anti Nuclear Factor Kappa B, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/pm36881363-84-45-52?v=Proteintech
Average 97 stars, based on 1 article reviews
rabbit anti nuclear factor kappa b - by Bioz Stars, 2026-08
97/100 stars
  Buy from Supplier
rabbit antinos  (Boster Bio)
90
Boster Bio rabbit antinos
Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB <t>p65</t> nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.
Rabbit Antinos, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/pm23007408-76-12-16?v=Boster+Bio
Average 90 stars, based on 1 article reviews
rabbit antinos - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
rabbit antinf κb p65  (Bioss)
94
Bioss rabbit antinf κb p65
Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB <t>p65</t> nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.
Rabbit Antinf κb P65, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/ppr0536925-250-31-41?v=Bioss
Average 94 stars, based on 1 article reviews
rabbit antinf κb p65 - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
rabbit antin antibody  (GeneTex)
90
GeneTex rabbit antin antibody
Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB <t>p65</t> nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.
Rabbit Antin Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antinos+polyclonal+ab/pm39900678-110-16-19?v=GeneTex
Average 90 stars, based on 1 article reviews
rabbit antin antibody - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB p65 nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.

Journal: International journal of molecular medicine

Article Title: Co-culture supernatants from Vibrio vulnificus-infected INT-407 cells induce IL-8 production in intestinal epithelial cells: crucial role of V. vulnificus rtxE.

doi: 10.3892/ijmm_00000510

Figure Lengend Snippet: Figure 6. Effects of co-culture supernatants from rtxE CT V. vulnificus-infected INT-407 cells on NF-κB DNA activation and IκB· phosphorylation. (A) Human intestinal epithelial cells were transiently co-transfected with the NF-κB minimal promoter/luciferase construct and pRL-TK reporter vector, followed by 12 or 24 h of treatment with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. Afterwards, the cells were washed with PBS and the luciferase activity was determined. The results were normalized by Renilla luciferase activity, and are expressed as the relative fold induction. The data are representative of 3 independent experiments. The data are expressed as the means ± standard errors (n=3). *P<0.01, relative to the group treated with co-culture supernatants from WT V. vulnificus-infected INT-407 cells at 24 h. **P<0.05, relative to the group treated with co-culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells at 24 h. (B) Human intestinal epithelial cells were treated for 1-3 h with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. NF-κB DNA binding activity in the nuclear extracts was assessed via EMSA. S (specific) and NS (non-specific) indicate the presence of a 50-fold excess of specific oligonucleotide (NF-κB) and non-specific oligonucleotide (CRE-containing oligonucleotide), respectively. (C) Human intestinal epithelial cells were treated with co-culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cell lysates were prepared and analyzed by Western blot analysis using anti-IκB·, anti-pIκB· and ß-actin antibodies. The data are representative of 3 independent experiments. (D) Reduced NF-κB p65 nuclear translocation in human intestinal epithelial cells treated with co- culture supernatants from rtxE MT V. vulnificus-infected INT-407 cells. Human intestinal epithelial cells grown in 24-well plates were treated for 3 h with co- culture supernatants from WT, rtxE MT, or rtxE CT V. vulnificus-infected INT-407 cells. The cells were washed, fixed, permeabilized, and stained with anti- p65 polyclonal antibody followed by staining with FITC-conjugated secondary antibody (green) or rhodamine phalloidin (red; actin staining). NF-κB p65-stained cells were merged with rhodamine phalloidin actin staining. The data are representative of 3 independent experiments. WT, wild-type; rtxE MT, rtxE mutant; rtxE CT, rtxE-complemented.

Article Snippet: The cells were incubated with a rabbit antiNF-κB p65 antibody (diluted 1:200) for 1 h at room temperature, and then incubated with fluorescein isothiocyanateconjugated secondary antibody (Santa Cruz Biotechnology).

Techniques: Co-Culture Assay, Infection, Activation Assay, Phospho-proteomics, Transfection, Luciferase, Construct, Plasmid Preparation, Activity Assay, Binding Assay, Western Blot, Translocation Assay, Staining, Mutagenesis